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Optimization of real-time PCR method for quantification of human mtDNA in clinical samples
LOSKOT, Martin
This bachelor thesis dealing with measuring mitochondrial DNA (mtDNA) and how is it important to pathological conditions. Mitochondria are one of the cell organelles in eukaryotic cells. They are important to energy metabolism, aging process, and apoptosis. In current studies mitochondrial dysfunctions cause mitochondrial diseases and common illnesses, for example cardiovascular diseases, brain pathology or cancer. Studies show, that copy number of mtDNA correlates with state of health and aging process. Copy number of mtDNA is often measured by ratio of mtDNA to nuclear DNA. Measuring mtDNA of peripheral blood is appropriate indicators for mitochondrial functions because decreased copy number of mtDNA correlates with decreased function of mitochondria. We don't know, how copy number of mtDNA influence on illnesses, because it doesn't still clarify. However, studies show, that copy number of mtDNA can be biomarker of control of state of health, when we take real time measurement. In the practical part of this bachelor thesis describe preparation of samples from buccal swabs and from peripheral blood. It also describes method of measuring mtDNA by real-time PCR. There is calculation of copy number of mtDNA and evaluation of our results too. As part of the measurement optimization are described differences between two methods for diagnostic of relative copy number of mtDNA.
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The state of health of people in the Czech Republic
Gvoždiaková, Alžběta ; Langhamrová, Jitka (advisor) ; Krbcová, Daniela (referee)
The bachelor thesis focuses on the state of health of people in the Czech Republic. Health is one of the most important values in human life so that is why we need to pay it increased attention. The thesis concentrates on three specifically areas. The first, theoretical part concerns the definition of key terms relate with the state of health such as health, the determinant of health, the types of prevention, the analysis and monitoring of the state of health. The next part engages to European Health Examination Survey (EHES) that was organised in the Czech Republic in 2014. The survey focuses primarily on risk factors of cardiovascular diseases such as hypertension, diabetes and overweight, and lifestyle risk factors such as smoking, physical activity and eating habits. The thesis aim at a detailed discription of the measured results of this survey. The last part focuses at the development of selected demographic indicators of the state of health of people.
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The influence of nutrition on a children's state of health
Křivanová, Daniela ; Mlejnková, Lena (advisor) ; Indrová, Jarmila (referee)
The aim of this bachelor thesis is to analyse the food consumption in the Czech Republic in the context of the development of children's state of health. The theoretical part deals with the nutrition in general and its importance for a children's organism. The practical part is divided into two sections. The first part analyses the food consumption in the Czech Republic. The second part is dedicated to the development of the most frequent diseases connecting with the nutrition.
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Health social aspects of Czech professional soildiers serving in foreign mission.
KOVAŘÍKOVÁ, Elena
I sought to outline the issue of the missions and I focused on life situations of soldiers returning home from the missions, both in terms of health and social aspects. The introduction outlines the objective of thesis. In the theoretical part I have described the basic terms, with an overview of the issue of missions and adaptation of professional soldiers after returning to the home country. In the final part I have explained the methodology and process of the quantitative research. The thesis is concluded with a presentation and interpretation of results.
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